ADME-Toxicology

PBMCs For Translational Research

HTCR-Services offers human PBMCs isolated with optimized methods that result in high cell viability and purity. PBMCs consists of lymphocytes (T cells, B cells and NK cells) and monocytes isolated from peripheral blood using density gradient centrifugation followed by red blood cell lysis. Our isolated cells can be shipped overnight for immediate use or cryopreserved. In addition, researchers can opt for accompanying donor data when required for their studies. All our collections are carried out after written informed consent following protocols approved by IRBs.

Application Summary

PBMCs can be used for many purposes such as drug discovery, assay validation and development or mechanistic studies. Some specific applications are as followed:

• Cytotoxicity assays
• Toxicological studies
• Biomarker discovery
• Vaccine development
• Proliferation assays
• ELISPOT
• Cytokine bead arrays/ protein arrays
• Immunogenicity assays
• Mixed lymphocyte reactions
• Plaque assays
• Cell population characterization
• Intracytoplasmic cytokine staining
• Transmigration assays

Characterization

Isolated PBMCs have high purity (98 ± 1% CD45+ cells) and high viability (98 ± 1%). Cryo-preserved PBMCs maintain a high viability after thawing at 92 ± 1%.

Related Products

• Diseased (e.g. cancer, inflammed, fibrotic or cirrhotic tissue) or macroscopically healthy tissue from the resection margin of the same patient after elective surgery.
• Biofluid specimens e.g. plasma, serum, urine, ascites from the same patient.
• Isolated cells from the same patient, e.g. hepatocytes, liver non-parenchymal cell-fraction (Kupffer cells, hepatic stellate cells, liver sinusoidal endothelial cells), cancer cells or intestinal crypts.
• Basic donor data set: ICD code, age, sex, BMI, infectious disease tests (HIV, HBV, HCV and Covid-19), tumor classification, histopathological diagnosis, tissue type (normal or diseased), organ type.
• Extended donor data set: operation date, OPS-code, ethnicity if available, ASA classification, pathological analysis, other microscopic findings, anamnesis, long-term medication, tumor therapy (chemotherapy/radiotherapy), blood laboratory values, tumor markers if available, ischemic time during collection.

Customized Services

HTCR-Services offers customized collections for prospective studies with specific inclusion and exclusion criteria. Please contact us if you would like to obtain additional contract research services.

Human NPCs For Research

HTCR-Services offers freshly-collected primary human liver NPCs isolated with a two-step collagenase perfusion method followed by differential centrifugation and a red blood cell lysis step. Primary NPCs interact with hepatocytes and modulate overall liver response and drug toxicity and hence are important for building complex representative co-cultures. For researchers our isolated NPCs can be shipped overnight immediately after isolation for your experiments starting the next morning. The received cell suspension containing Kupffer cells, liver sinusoidal epithelial cells and stellate cells, can be used immediately for co-culture or for subsequent purification of a particular cell type. In addition, researchers can opt for accompanying donor data when required for their studies. All our collections are carried out after written informed consent following protocols approved by IRBs.

Application Summary

Human NPCs together with hepatocytes can be used to establish in vitro co-culture models that allow modelling of interactions between different cell types. The NPC suspension can also be further purified for single cell types for various research interests. Some specific applications are as follows:

• Cellular toxicity
• Drug-drug interaction assays
• Drug uptake
• Drug transport kinetics
• Enzyme induction
• Metabolic profiling
• Metabolic stability
• Modeling diseases

Characterization

Fluorescence-activated cell sorting (FACS) shows that the NPC fraction contains CD31+/CD146+ endothelial cells, CD68+ Kupffer cells and Desmin+ hepatic stellate cells.

The FACS results are corroborated by red immunofluorescent staining of the various NPC markers.

Related Products

• Diseased (e.g. cancer, inflammed, fibrotic or cirrhotic tissue) or macroscopically healthy tissue from the resection margin of the same patient after elective surgery.
• Biofluid specimens e.g. plasma, serum, urine, ascites from the same patient.
• Isolated cells from the same patient, e.g. hepatocytes, liver non-parenchymal cell-fraction (Kupffer cells, hepatic stellate cells, liver sinusoidal endothelial cells), cancer cells or intestinal crypts.
• Basic donor data set: ICD code, age, sex, BMI, infectious disease tests (HIV, HBV, HCV and Covid-19), tumor classification, histopathological diagnosis, tissue type (normal or diseased), organ type.
• Extended donor data set: operation date, OPS-code, ethnicity if available, ASA classification, pathological analysis, other microscopic findings, anamnesis, long-term medication, tumor therapy (chemotherapy/radiotherapy), blood laboratory values, tumor markers if available, ischemic time during collection.